Generator

Part:BBa_K2055369:Design

Designed by: Suria Itzel Morales Guzm�n   Group: iGEM16_Tec-Monterrey   (2016-10-12)


Na(+)/H(+) antiporter NhaA generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 415
    Illegal BamHI site found at 1120
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 382
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence is optimized for its expression in C. violaceum and could be tested as a potential selective marker for C. violaceum. This construct is regulated by a weak constitutive promoter and its objective is to augment bacterial resistance to alkaline pH.



Source

The sequence was obtained from Uniprot P13738. The origin for this sequence is E. Coli.

References